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Two primary applications are Cell Rupture and Homogenisation.
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Cell Rupture
Stansted Fluid Power general experience in this field of application
is that a single stage homogenisation process is adequate and that the
style of the valve makes minimal difference to the effectiveness of the
disruption. It is our observation that as percentage cell content increases
in the suspension that the disruption yield is likely to drop.
| Cell Rupture | ||
| Soft Mammalian Cells (i.e. Lymphocytes) | 1 to 15 MPa | |
| Organelles & Mitrochrondia | 1 to 15 MPa | |
| Parasites (Typanosomes) | to 15 MPa | |
| Sperm | 1 to 35 MPa | |
| Nematodes | 1 to 35 MPa | |
| E.coli | 30 to 140 MPa | |
| Mammalian Tissue | 70 to 150 MPa | |
| Vegetative Cells and Cyanobacteria | 35 to 150 MPa | |
| Yeast | 100 to 250 MPa | |
| Stratum Corneum | 140 to 200 MPa | |
| DNA | 100 to 250 MPa | |
| Hard Bacteria (staphylococcus, streptococcus) | 150 to 320 MPa | |
| Homogenisation | ||
| Liposomes | 20 to 150 MPa | |
| Microemulsions and nanoparticles | 20 to 350 MPa | |
| Deagglomeration | 30 to 350 MPa | |
| Lipid Nanoparticles | 50 to 200 MPa * | |
| Microbial inactivation food preservation | 200 to 400 MPa |
*Temperatures to 80°C (lipid melt temperature) may be required, for some applications.
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